Sep 012014
 

Scouring the planet for breeding solutions

Bindiganavile Vivek

Bindiganavile Vivek

Bindiganavile Vivek (pictured) is a maize breeder working at the International Maize and Wheat Improvement Center (CIMMYT), based in Hyderabad, India. For the past five years, Vivek and his team have been developing drought-tolerant germplasm for Asia using relatively new molecular-breeding approaches – marker-assisted recurrent selection (MARS), applied in a genomewide selection (GWS) mode. Their work in the Asian Maize Drought-Tolerance (AMDROUT) project is implemented through GCP’s Maize Research Initiative, with Vivek as the AMDROUT Principal Investigator.

Driven by consumer demand for drought-tolerant maize varieties in Asia, the AMDROUT research team has focussed on finding suitable drought-tolerant donors from Africa and Mexico. Most of these donors are white-seeded, yet in Asia, market and consumer preferences predominantly favour yellow-seeded maize. Moreover, maize varieties are very site-specific and this poses yet another challenge. Clearly, breeding is needed for any new target environments, all the while also with an eye on pronounced market and consumer preferences.

(1) Amazing maize and its maze of colour. Maize comes in many colours, hues and shapes. (2) Steeped in saffron: from this marvellous maize mix and mosaic, the Asian market favours yellow maize.

(1) Amazing maize and its maze of colour. Maize comes in many colours and hues. (2) Steeped in saffron: from this marvellous maize mix and mosaic, the flavour in Asia favours yellow maize.

Stalked by drought, tough to catch, but still the next big thing

Around 80 per cent of the 19 million hectares of maize in South and Southeast Asia is grown under rainfed conditions, and is therefore susceptible to drought, when rains fail. Tackling drought can therefore provide excellent returns to rainfed maize research and development investments. As we shall see later, Vivek and his team have already made significant progress in developing drought-tolerant maize.

Drough in Asia_Vivek slide_GRM 2013_w

The stark reality of drought is illustrated in this warning sign on a desiccated drought-scorched landscape, showing the severity of drought in Asia

But they are after a tough target: drought tolerance is dodgy since it is a highly polygenic trait, making it difficult for plant scientists to pinpoint genes for the trait (see this video with an example from rice in Africa). In other words, to make a plant drought-tolerant, many genes have to be incorporated into a new variety. As one would expect, the degree of difficulty is directly proportional to the number of genes involved. In the private-sector seed industry, MARS  (PDF) has been successfully used in achieving rapid progress towards high grain yield under optimal growth conditions. Therefore, a similar approach could be used to speed up the process of introducing drought tolerance into Asian crops – the reason why the technique is now being used by this project.

AMDROUT Meeting Penang Dec2010_w

More than India: the AMDROUT project also comprises research teams in China, Indonesia, Thailand, The Philippines and Vietnam. In this photo taken during the December 2010 annual project meeting in Penang, Malaysia, the AMDROUT team assessed the progress made by each country team, and  team members were trained in data management and drought phenotyping. They also realised that there was a need for more training in genomic selection, and did something about it, as we shall see in the next photo. Pictured here, left to right: Luo Liming, Tan jing Li, Villamor Ladia, V Vengadessan, Muhammad Adnan, Le Quy Kha, Pichet Grudloyma, Vivek, IS Singh, Dan Jeffers (back), Eureka Ocampo (front), Amara Traisiri and Van Vuong.

The rise of maize: clear chicken-and-egg sequence…

Vivek says that the area used for growing maize in India has expanded rapidly in recent years. In some areas, maize is in fact displacing sorghum and rice. And the maize juggernaut rolls beyond India to South and Southeast Asia. In Vietnam, for example, the government is actively promoting the expansion of  maize acreage, again displacing rice. Other countries involved in the push for maize include China, Indonesia and The Philippines.

So what’s driving this shift in cropping to modern drought-tolerant maize? The curious answer to this question lies in food-chain dynamics. According to Vivek, the dramatic increase in demand for meat – particularly poultry – is the driver, with 70 percent of maize produced going to animal feed, and 70 percent of that going into the poultry sector alone.

GCP gave us a good start… the AMDROUT project laid the foundation for other CIMMYT projects”

 Show and tell: posting and sharing dividends

As GCP approaches its sunset in December 2014, Vivek reports that all the AMDROUT milestones have been achieved. Good progress has been made in developing early-generation yellow drought-tolerant inbred lines. The use of MARS by the team – something of a first in the public sector – has proved to be useful. In addition, regional scientists have benefitted from broad training from experts on breeding trial evaluation and genomic selection (photo-story on continuous capacity-building). “GCP gave us a good start. We now need to expand and build on this,” says Vivek.

AMDROUT trainees at Cambridge_w

AMDROUT calls in on Cambridge for capacity building. AMDROUT country partners were at Cambridge University, UK, in March 2013, for training in quantitative genetics, genomic selection and association mapping. This was a second training session for the team, the first having been September 2012 in India.
Pictured here, left to right – front row: Sri Sunarti, Neni Iriany, Hongmei Chen;
middle row: Ian Mackay (Cambridge), Muhammad Azrai, Le Quy Kha, Artemio Salazar;
back row: Roy Efendy, Alison Bentley (who helped organise, run and teach on the course, alongside Ian) and Suriphat Thaitad.AMDROUT country partners are from China’s Yunnan Academy of Agricultural Sciences (YAAS); the Indonesian Cereals Research Institute (ICERI); the Institute of Plant Breeding at the Unversity of Philppines at Los Baños (UPLB); Thailand’s Nakhon Sawan Field Crops Research Center (NSFCRC); Vietnam’s National Maize Research Institute (NMRI); and private-sector seed companies in India, such as Krishidhan Seeds.Curious on who proposed to whom for this AMDROUT–Cambridge get-together? We have the answer: a Cambridge callout announced the training, and AMDROUT answered by calling in, since course topics were directly relevant to AMDROUT’s research approach. 

 

 

According to Vivek, the AMDROUT project laid the foundation for other CIMMYT projects  such as the Affordable, Accessible, Asian (AAA) Drought-Tolerant Maize (popularly known as the ‘Triple-A project’) funded by the Syngenta Foundation for Sustainable Agriculture. This Triple-A project is building on the success of AMDROUT, developing yet more germplasm for drought tolerance, and going further down the road to develop hybrids.

 

Outputs from the AMDROUT project will be further refined, tested and deployed through other projects”

Increasing connections, and further into the future

Partly through GCP’s Integrated Breeding Platform (IBP), another area of success has been in informatics. Several systems such as the Integrated Breeding FieldBook, the database Maize Finder and the International Maize Information System (IMIS) now complement each other, and allow for an integrated data system.

There is now also an International Maize Consortium for Asia (IMIC–Asia), coordinated by CIMMYT, comprising a group of 30 commercial companies (ranging from small to large; local to transnational). Through this consortium, CIMMYT is developing maize hybrids for specific environmental conditions, including drought. IMIC–Asia will channel and deploy the germplasms produced by AMDROUT and other projects, with a view to assuring impact in farmers’ fields.

Overall, Vivek’s experience with GCP has been very positive, with the funding allowing him to focus on the agreed milestones, but with adaptations along the way when need arose: Vivek says that GCP was open and flexible regarding necessary mid-course corrections that the team needed to make in their research.

But what next with GCP coming to a close? Outputs from the AMDROUT project will be further refined, tested and deployed through other projects such as Triple A, thus assuring product  sustainability and delivery after GCP winds up.

Links

As our Maize Research Initiative does not have a Product Delivery Coordinator, Vivek graciously stepped in to coordinate the maize research group at our General Research Meeting in 2013, for which we thank him yet again. Below are slides summing up the products from this research, and the status of the projects then.

Jun 202012
 

Breathing life into support services

By addressing the needs at the heart of quality agricultural research, right there on the station, GCP was the first to cotton on to a crucial missing link between researcher, research station, and support services.” – Hannibal Muhtar (pictured)

“One thing that really energises me,“ enthuses GCP Consultant Hannibal Muhtar, “is seeing people understand why they need to do the work, and being given the chance to do the how.”

And so was born another wonderfully fruitful GCP collaboration. Hannibal, who describes the assignment as “a breath of fresh air,” was asked to identify, together with GCP project Principal Investigators, African research sites of ongoing or potential GCP Research Initiative projects where effective scientific research might be hampered by significant gaps in one fundamental area: infrastructure, equipment and support services. As at June 2012, 19 sites had been selected.

Meet Hannibal Muhtar (audio clip)

Embarking on the voyage to change, storms ‘n’ all
In 2010 and 2011, Hannibal visited these stations, meeting staff at all levels and functions, for an in-depth analyses and appropriate recommendations to assure high-quality field evaluations for GCP-funded projects. With funding from GCP’s Integrated Breeding Platform (IBP), and with the openness, commitment and energy of station staff to implement these recommendations, the efforts are, starting to bear fruit.

Photos: AgCommons

Flashback to 2010. Photo 1: Hannibal (centre) at a planning session at Sega, Western Kenya, with Samuel Gudu of Moi University  (right) and Onkware Augustino (left). Photo 2: Similarly, at Tanzania’s Agricultural Research Institute, Naliendele, with Omari Mponda (right).

But it has not all been smooth sailing, and the storms encountered along en route should not be underestimated.

Weeds, wear and tear, and a walk on the wild side
“The real challenge,” says GCP’s Director of Research, Xavier Delannay (pictured), “is not in the science, but rather in the real nuts-and-bolts of getting the work done in local field conditions.” The obstacles, are often mundane – missing or faulty, weather stations or irrigation systems; weed-infested fields ravaged, or poor drainage, for example. Yet such factors compromise brilliant research. Take unfenced plots for example – equipment gets stolen, and animals roam freely.

Getting down to the brass tacks of local empowerment, and aiming higher
The overarching objective is, in Xavier’s words, “The effective running of local stations, for facilitating local research, improving local crops, and ultimately leading to empowerment and self-reliance of local farming communities.”

In tackling the matter, Hannibal employed a multi-faceted customised approach, based on the needs of each site, for both equipment and training for technicians, tractor operators and station managers. The dedication of the managers to both learn and continue these efforts after the training was particularly gratifying, since it assures sustainability.

“At the end of the day, it’s about achieving food security and improving livelihoods… which pave the way for healthy families and profitable agriculture,” concludes Hannibal.

Lights, curtain… action!
Much like in theatre, with all the ‘props’ in place, field trials are now performing well, thanks to streamlined ‘backstage’ support. Hannibal likens the positive feedback from the partners he has worked with to “A glass of cold water, after a long day in the sun!”

“With proper infrastructure in place, and with research station staff duly equipped with the hands-on expertise and practical know-how to utilise and apply this infrastructure and training, we’re now seeing field experiments being conducted as they should be, and getting good-quality phenotyping data as a result,” says Xavier. “Moreover,” he continues, “by providing glass-houses or the capacity to irrigate in the dry season, we are enabling breeders to accelerate their breeding cycles, so that they can work all year round, rather than having to wait until the rain comes.”
Examples include sites in Kenya, Mali and Nigeria.

The missing link
As the nuts-and-bolts begin to fall in place for, Hannibal reveals: “By addressing the needs at the heart of quality agricultural research, right there on the station, GCP was the first to cotton on to a crucial missing link between researcher, research station, and support services.”

…and yet another missing link…
But the job is not quite done. One crucial gap is the sensitisation of upper management – those at the helm of national research institutes and research station Directors – to support and sustain infrastructure, training and related services. In some cases, costs could be easily met by utilising a priceless asset that most institutes already have, and which they could put to greater us – land and a controlled environment.

Upper management needs to be actively on board. “A research institute should work like a good sewing machine,” says Hannibal. “All well-oiled, all parts working well, and everybody knowing what they need to do.”

In the meantime, however, results from the field suggest that researchers in GCP projects are already reaping the benefits from improved infrastructure and support services, and are already off to a good start.

The ‘stage’ is therefore set: ‘backstage’ and ‘props’ are well primed, performance trials are acting like they should, and the ‘theatre directors’ have an eye on sustainability after GCP’s final curtain call in 2014.

So, long may the show go on, with a cautionary word, however, to continually seek ways to not only maintain but also enhance performance!

Want more details? Read the extended version of this story

Relevant links

  • PODCASTS: You can also listen to Hannibal, by tuning into Episode 2 for the entire interview, or zooming in on your particular area of interest in the mini-podcasts labelled Episodes 2.1 to 2.7 c here.
  • Capacity building
  • Research Initiatives
  • Integrated Breeding Platform website
Jun 202012
 

Breathing life into support services

By addressing the needs at the heart of quality agricultural research, right there on the station, GCP was the first to cotton on to a crucial missing link between researcher, research station, and support services.” – Hannibal Muhtar

Want to cut to the chase and only need the bare bones of this story? Skip over to the short version

“One thing that really energises me,” enthuses GCP Consultant Hannibal Muhtar, “is seeing people understand why they need to do the work, and being given the chance to do the how.” And so was born another wonderfully fruitful GCP collaboration. Hannibal, who describes the assignment as “a breath of fresh air,” was asked to identify, together with GCP project Principal Investigators, African research sites of ongoing or potential GCP Research Initiative projects where effective scientific research might be hampered by significant gaps in one fundamental area: infrastructure, equipment and support services.

Meet Hannibal Muhtar (Audio clip)

As at June 2012, the 19 sites selected were:

Burkina Faso – L’Institut de l’environnement et de recherches agricoles sites at :
1.  Banfora
2.  Farako-Bâ Regional Centre
Ethiopia
3.  Hawassa Agricultural Research Station
4.  The Southern Agricultural Research Institute
Ghana – Council for Scientific and Industrial Research, Crops Research Institute sites at:
5.  Kumasi
6.  Tamale
Kenya
7.    Moi University (site 1)
8.    Moi University (site 2)
9.    Egerton University (Njoro site)
10.    Egerton University (Koibatek Farmers Training Centre)
Mali – L’Institut d’Économie Rurale sites at:
11.    Sotuba
12.    Cinzana
13.    Longrola
Niger – ICRISAT site
14.    Sadore
Nigeria
15.    National Cereals Research Institute
National Root Crops Research Institute sites at:
16.    Umudike
17.    Kano
Tanzania – Agricultural Research Institute at:
18.    Naliendele
19.    Mtwara

Flashback to 2010. Picture on the left: Hannibal at a planning session at Sega, Western Kenya, with Samuel Gudu and  Onkware Augustino. Picture on the right: Similarly, at Naliendele, in Tanzania with Omari Mponda.

Flashback to 2010. Picture on the left: Hannibal at a planning session at Sega, Western Kenya, with Samuel Gudu and  Onkware Augustino. Picture on the right: Similarly, at Naliendele, in Tanzania with Omari Mponda.

Embarking on the voyage to change, storms ‘n’ all
Hannibal, armed with years of practical experience in the application of engineering sciences in agriculture and developing countries, as well as an attentive ear to the real needs of researchers, embarked on a series of visits to these research stations in 2010 and 2011, meeting with staff of all levels, departments and functions, carrying out in-depth analyses and draw up concrete recommendations for infrastructure and support service investments for each of the sites so that good-quality field evaluations (‘phenotyping’ in ‘breeder-speak) of GCP-funded projects could be conducted. Thanks to funding from GCP’s Integrated Breeding Platform (IBP), and to the openness, commitment and energy of research staff on the ground to implement these recommendations, the efforts of multiple cross-cutting partnerships across Sub-Saharan Africa are, in 2012, starting to bear fruit. But it has not all been smooth sailing, and the storms encountered along the way to reach this end goal should not be underestimated.

Weeds, wear and tear, and a walk on the wild side
The obstacles, says GCP’s Director of Research, Xavier Delannay (pictured, can often be mundane in nature – a  lack of or faulty weather stations or irrigation systems, or fields ravaged by weeds or drainage problems and in dire need of rehabilitation, for example. Yet such factors compromise brilliant research. A simple lack of fencing, Xavier and Hannibal expound, commonly results not only in equipment being stolen, but also in roaming cattle and wild animals – boars, monkeys, hippopotamus and hyena, to name but a few – stomping over precious experiment sites and posing serious threats to field staff safety. “The real challenge lies not in the science, but rather in the real nuts-and-bolts of getting the work done in local field conditions,” he explains.’’

Hannibal concurs: “If GCP had not invested in these research support infrastructure and services, then their investment in research would have been in vain. Tools and services must be in place as and when needed, and in good working order. Tractors must be able to plough when they should plough.’’

But a critical change is also needed in mindset and budgeting. ‘’The word ‘maintenance,’’’ a Senegalese partner commented to Hannibal, describing his institute, “does not exist in our vocabulary and is not a line-item on our budget.”

The problem then is not always about limited funds but rather much more on how the funds available are budgeted, excluding the all-essential support services.

Getting down to the brass tacks of local empowerment, and aiming higher
Multi-lingual and fluent in English, Arabic and French, Hannibal employed a multi-faceted customised approach, based on the needs of each site, be it sharing his tricks-of-the-trade and improvising local solutions, or guiding researchers in identifying their specific needs, as well as on where and how to request equipment, just to mention a few examples. In other cases he would teach local station managers to build and apply simple yet revolutionary tools such as land-levellers (referred to as ‘floats’ in industrial-speak), as well as row-markers for more uniform spacing between rows and plants in the field.

In addition, he would organise a training workshops in either English or French, with different content for technicians, machine operators and station managers. The dedication demonstrated by this latter group to both learn and continue these efforts after the training was particularly pertinent for ensuring the long-term sustainability of the investments.

A colourful menu of options, then, for achieving one common overarching objective, which, as summarised neatly by Xavier, is: “The effective running of local experiment stations, for facilitating local research, improving local crops, and ultimately leading to empowerment and self-reliance of local farming communities.”

“At the end of the day, it’s about achieving food security and improving livelihoods,” Hannibal emphasises. Looking back at some of the research stations that are now well-equipped and are being managed well, and the improved crop varieties being produced and projected, Hannibal highlights the “harmonious chain” triggered as a result: “Food security and better livelihoods pave way for healthy, well-fed families, and agriculture growing beyond subsistence into an economic activity,” Hannibal concludes.

Lights, curtain… ACTION!
Much like in theatre, with all the ‘props’ in place, Hannibal reports that field trials are now performing well, thanks to the all-important ‘backstage’ support service elements being in good shape. Hannibal likens the positive feedback from the partners he has worked with to “A glass of cold water, after a long day in the sun!”

And there’s a beautiful simplicity to the impacts described: “With proper infrastructure in place, and with research station staff duly equipped with the hands-on expertise and practical know-how to utilise and apply this infrastructure and training, we’re now seeing field experiments being conducted as they should be, and getting good-quality phenotyping data as a result,” says Xavier. “Moreover,” he continues, “by providing glass-houses or the capacity to irrigate in the dry season, we are enabling breeders to accelerate their breeding cycles, so that they can work all year round, rather than having to wait until the rain comes.” Sites hosting GCP projects on rice in Nigeria, as well as on sorghum and rice in Mali, are just a few examples of those enjoying off-season work thanks to new irrigation systems.

Similar good news is expected soon for cassava in Ghana and in northern Nigeria. And yet more good news: in some cases, the impacts have not been limited to the trials, or even to the research trials and stations alone, as Xavier highlights with an example from Kenya: “The establishment of an irrigation system on a plot at Koibatek Farmer Training Centre – a partner of Egerton University – yielded excellent results for chickpea experiments. We emphasised that we did not want the equipment to be ‘bracketed’ exclusively for science and experiments. So, it was also used to train staff and farmers from the local community as well. This was greatly appreciated.”

Seeing the nuts-and-bolts now firmly in place for the majority of the sites visited, Hannibal believes GCP has facilitated a pioneering approach to local capacity building: “By addressing the needs at the heart of quality agricultural research, right there on the station, GCP was the first to cotton on to a crucial missing link between researcher, research station, and support services,” he reveals.

…Another missing link…
But the job is not quite done. One crucial gap is the sensitisation of upper management – those at the helm of national research institutes and research station Directors – to support and sustain infrastructure, training and related services. In some cases, costs could be easily met by utilising a priceless asset that most institutes already have, and which they could put to greater us – land and a controlled environment.

Upper management needs to be actively on board. “A research institute should work like a good sewing machine,” says Hannibal. “All well-oiled, all parts working well, and everybody knowing what they need to do.”

In the meantime, however, results from the field suggest that researchers in GCP projects are already reaping the benefits from improved infrastructure and support services, and are already off to a good start.

The stage is therefore set: backstage and props are well primed, performance trials are acting like they should, and the ‘theatre directors’ have an eye on sustainability after GCP’s final curtain call in 2014.

So, long may the show go on, with a cautionary word, however, to continually seek ways to not only maintain but also enhance performance!

Relevant links

  • PODCASTS: You can also listen to Hannibal, by tuning into Episode 2 for the entire interview, or zooming in on your particular area of interest in the mini-podcasts labelled Episodes 2.1 to 2.7 c here.
  • Capacity building
  • Research Initiatives
  • Integrated Breeding Platform website

 

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