Jan 122015
 
James profile

James Gethi and one of the crops closest to his heart – maize. He also has a soft spot for hardy crop varieties that survive harsh and unforgiving drylands, such as Machakos, Kenya, where this June 2011 photo of him with drought-tolerant KARI maize was taken.

As we tell our closing stories on our Sunset Blog, in parallel, we’re also catching up on the backlog of stories still in our store from the time GCP was a going concern. Our next stop is Kenya, and the narrative below is from 2012, but don’t go away as it is an evergreen – a tale that can be told at any time, as it remains fresh as ever. At that time, and for the duration of the partnership with GCP, the Food Crops Research Institute of the Kenya Agricultural and Livestock Research Organisation (KALRO) was then known as the Kenya Agricultural Research Institute (KARI), and we shall therefore stay with this previous name in the story. KARI was also the the name of the Kenyan institute at the time when James Gethi (pictured) left for a sabbatical at the International Maize and Wheat Improvement Center (CIMMYT by its Spanish acronym). On to the story then, and please remember we’re travelling back in time to the year 2012. 

“I got into science by chance, for the fun of it,” muses James, maize breeder and former GCP scientist “With agricultural school promising a flight to overfly the country’s agricultural areas– this was an interesting prospect for a village guy. ‘This could be fun’, I thought!”

And it turned out to be a chance well worth taking.  His first step was getting the requisite education. And so he armed himself with a BSc in Agriculture from the University of Nairobi, Kenya, topped with a Master’s and PhD in Plant Breeding from the University of Alberta (Canada) and Cornell University (USA), respectively. Beyond academics, in the course of his crop science career, James has developed 13 crop varieties, that included maize and cassava, published papers in numerous peer-reviewed papers (including the 2003 prize for Best paper in the field of crop science in the prestigious Crop Science journal. And in leadership, James headed the national maize research programme in his native Kenya. These are just a few of the achievements James has garnered in the course of his career, traversing  and transcending not only the geographical frontiers initially in his sights, but also scientific ones, reaching professional heights that perhaps his younger self might never have dreamt possible.

As a Research Officer at KARI, a typical day sees James juggling his time between hands-on research (developing maize varieties resistant to drought, field and storage pests) and project administration, coordinating public–private partnerships and the maize research programme at both institutional and country level. What motivates the man shouldering much of the responsibility for the buoyancy of his nation’s staple crop? James explains, “Making a difference by providing solutions to farmers. That’s my passion and that’s what makes me get up in the morning and go to work. It’s hugely satisfying!”

Without GCP, I would not be where I am today as a scientist… [it] gave me a chance to work with the best of the best worldwide… You develop bonds and understanding that last well beyond the life of the projects.”

Rapid transitions: trainee to trainer to leader
It was this passion and unequivocal dedication to his vocation – not to mention a healthy dollop of talent – that GCP was quick to recognise back in 2004, when James first climbed aboard the GCP ship. Like a duck to water, he proceeded to engage in all manner of GCP projects and related activities, steadily climbing the ranks from project collaborator to co-Principal Investigator and, finally, Principal Investigator in his own right, leading a maize drought phenotyping project. Along the way, he also secured GCP Capacity building à la carte and Genotyping Support Service grants to further the maize research he and his team were conducting.

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FLASHBACK: At a GCP drought phenotyping course in mid-2006 at Montpellier, France. (1) James (left) pays keen attention during one of the practical sessions. (2) In the spirit of “All work and no play, etc”, taking a break from the course to take in some of the sights with colleagues. Clearly, James, “the guy from the village” is anything but a dull boy! Next to James, second left, is BM Prasanna, currently leader of CIMMYT’s maize programme.

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From trainee to trainer and knowledge-sharer: James (behind the camera) training KARI staff on drought phenotyping in June 2009 at Machakos, in Kenya’s drylands.

The GCP experience, James reveals, has been immensely rewarding: “Without GCP, I would not be where I am today as a scientist,” he asserts. And on the opportunity to work with a capable crew beyond national borders, as opposed to operating as a solo traveller, he says: “GCP gave me a chance to work with the best of the best worldwide, and has opened up new opportunities and avenues for collaboration between developing-country researchers and advanced research institutes, creating and cementing links that were not so concrete before. This has shown that we don’t have to compete with one another; we can work together as partners to derive mutual benefits, finding solutions to problems much faster than we would have done working alone and apart from each other.”

The links James has in mind are not only tangible but also sustainable: “You develop bonds and understanding that last well beyond the life of the projects,” James enthuses, citing additional professional engagements (the African Centre for Crop Improvement in KwaZulu-Natal, South Africa, and the West Africa Centre for Crop Improvement, have both welcomed James and his team into their fold), as well as firm friendships with former GCP project colleagues as two key take-home benefits of his interaction with the Programme. These new personal and professional circles have fostered a happy home for dynamic debates on the latest news and views from the crop-science world, and the resultant healthy cross-fertilisation of ideas, James affirms.

Reflecting on what he describes as a ‘mentor’ role of GCP, and on the vital importance of capacity building in general, he continues: “By enhancing the ability of a scientist to collect germplasm, or to analyse that germplasm, or by providing training and tips on how to write a winning project proposal to get that far in the first place, you’re empowering scientists to make decisions on their own – decisions which make a difference in the lives of farmers. This is tremendous empowerment.”

Another potent tool, says James, is the software made available to him through GCP’s Integrated Breeding Platform (IBP), which is a handy resource package to dip into for – among other things – analysing data and selecting the right varieties at the right time. The next step for IBP, he feels, should be scaling up and aiming for outreach to the wider scientific community, forecasting that such a step could bring nothing but success: “The impacts could be enormous!” he projects, with a palpable and infectious enthusiasm.

People… don’t eat publications, they eat food… I’m not belittling knowledge, but we can do both”

Fast but not loose on the R&D continuum: double agent about?
For James, outreach and impacts are not limited to science alone. In parallel with his activities in upstream genetic science, James’ efforts are equally devoted to the needs of his other client base-–the development community and farmers. For this group, James’ focus is on putting tangible products on the table that will translate into higher crop yields and incomes for farmers. Yet whilst products from any highly complex scientific research project worth its salt are typically late bloomers, often years in the making on a slow burner as demanded by the classic linear R&D view that research must always precede development, adaptation and final adoption, James has been quick to recognise that actors in the world of development and the vulnerable communities they serve do not necessarily have this luxury of time.

 August 2008: a huge handful, and more where that came from in Kwale, Kenya. This farmer's healthy harvest came from KARI hybrids.

August 2008: a huge handful, and more where that came from in Kwale, Kenya. This farmer’s healthy harvest came from KARI hybrids.

His solution for this challenge? “Sitting where I sit, I realised from very early on that if I followed the traditional linear scientific approach, my development clients would not take it kindly if I still had no products for them within the three-year lifespan of the project. The challenge then was to deliver results for farmers without compromising or jeopardising their integrity or the science behind the product,” he recalls. In the project he refers to – a GCP-funded project to combat drought and disease in maize and rice – James applied a novel double-pronged approach to get around this seeming conundrum of the need for sound science on the one hand, and the need for rapid results for development on the other hand. Essentially, he simultaneously walked on both tracks of the research–development continuum.

The project – led by Rebecca Nelson of Cornell University and with collaborators including James’ team at KARI (leading the maize component), the International Rice Research Institute (IRRI), researchers in Asia, as well as other universities in USA – initially set out with the long-term goal of dissecting quantitative trait loci (QTLs) for rice and maize with a view to combating drought and disease in these crops. Once QTLs were dissected and gene crosses done, James and his team went about backcrossing these new lines to local parental lines, generating useful products in the short term. The results, particularly given the limited resources and time invested, have been impressive, with seven hybrid varieties developed for drylands and coastal regions having been released in Kenya by 2009, and commercialised from 2010.

James and his colleagues have applied the same innovative approach to other GCP projects, grappling to get a good grasp of the genetic basis of drought tolerance, whilst also generating intermediate products for practical use by farmers along the way. James believes this dual approach paves the way for a win-win situation: “People on the ground don’t eat publications, they eat food,” he says. “As we speak now, there are people out there who don’t know where their next meal will come from. I’m not belittling knowledge, but we can do both – boiled maize on the cob and publications on the boil. But let’s not stop at crop science  and knowledge dissemination – let’s move it to the next level, which means products,” he challenges, adding: “With GCP support, we were able do this, and reach our intended beneficiaries.”

It is perhaps this kind of vision and inherent instinct to play the long game that has taken James this far professionally, and that will no doubt also serve him well in the future.

As our conversation comes to a close, we ask James for a few pearls of wisdom for other young budding crop researchers eager to carve out an equally successful career path for themselves, James offers “Form positive links and collaborations with colleagues and peers. Never give up; never let challenges discourage you. Look for organisations where you can explore the limits of your imagination. Stay focused and aim high, and you’ll reach your goal.”

Upon completion of his ongoing sabbatical at CIMMYT in Zimbabwe, where he is currently working on seed systems, James plans to return to KARI, armed with fresh knowledge and ready to seize – with both hands – any promising collaborative opportunities that may come his way .

Certainly, prospects look plentiful for this ‘village lad’ in full flight, and who doesn’t look set to land any time soon!

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In full flight – Montpellier, Brazil, Benoni, Bangkok, Bamako, Hyderabad… our boy voyaged from the village to Brazil and back, and far beyond that. Sporting the t-shirt from GCP’s Annual Research Meeting in Brazil in 2006, which James attended, he also attended the same meeting the following year, in Benoni, South Africa, in 2007, when this photo was taken. James is a regular at these meetings which are the pinnacle on  GCP’s calendar (http://bit.ly/I9VfP4). But he always sings for his supper and is practically part of the ‘kitchen crew’, but just as comfortable in high company. For example, he was one of the keynote speakers at the 2011 General Research Meeting (see below).

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Jan 082015
 

Welcome to Brazil! Journey by road six hours northwest from Rio de Janeiro and you’ll arrive to Sete Lagoas,  a city whose name means ‘Seven Lagoons’ in Portuguese. Although cloistered in farmlands, the city is largely a commercial centre, but also the seat of Embrapa Milho e Sorgo, the nerve centre of EMBRAPA’s maize and sorghum research, and so could pass for the ‘sede’ (Portuguese for headquarters) of the these two cereals. EMBRAPA is the Portuguese acronym for Empresa Brasileira de Pesquisa Agropecuária; the Brazilian Agricultural Research Corporation. EMBRAPA is a GCP Consortium member, and contributed to the proposal that founded GCP.

Photo provided by J MagalhãesJurandir Magalhães (pictured), or Jura, as he likes to be referred to in informal settings such as our story today, is a cereal molecular geneticist who has been working at the Embrapa Milho e Sorgo centre since 2002. “The centre develops projects and research to produce, adapt and diffuse knowledge and technologies in maize and sorghum production by the efficient and rational use of natural resources,” Jura explains.

Such qualities are exactly what appeal to GCP, which has supported Jura as a Principal Investigator since 2004. Beyond science and on to governance and advisory issues, Jura is also EMBRAPA’s representative on the GCP Consortium Committee.

Home and away, on a journey of discovery in sorghum
Hailing from Belo Horizonte, Minas Gerais State, where he was born, Jura attended the Federal University of Viçosa in his home state. Upon completing his Master’s degree at the university in 1995, he proceeded to USA’s Cornell University in 1998 for his PhD, under the watchful eye of Leon Kochian, another GCP Principal Investigator.

Sorghum rainbow_A Borrell

No, it’s not photo-shopped. This Australian sorghum-and-double-rainbows shot is from Supa Snappa, Andy Borrell, also a GCP sorghum Principal Investigator. See http://bit.ly/1tBAOMW

At Cornell, Jura worked with Leon on identifying the genes associated with aluminium tolerance in sorghum. “At the time, genes associated with aluminium tolerance were known for cereals in the Triticeae family (wheat, barley and rye). But the same genes were not found in the Poaceae family (sorghum, rice and maize). This suggested that there were different aluminium-tolerance genes at play, so it was a really pioneering project.” Continuing with the Cornell team after his PhD, Jura worked with Leon to  map the location of a major aluminium-tolerance genetic ‘hotspot’ in sorghum, which the project team contracted to  AltSB  for short (aluminium-tolerance gene or locus in Sorghum bicolor). The mapping also marked the next chapter  of what was to be a long-term professional relationship for the pair.

Brazil beckons, joining GCP, leadership and enduring partnerships
But in between, Brazil broke in and beckoned her native son home. And so it was that in 2002, Jura packed his bags and accepted a position with EMBRAPA’s maize and sorghum research centre. And despite the geographical distance, it wasn’t long before he and Leon teamed up again. “When I left Cornell, Leon and I had finished mapping AltSB and we were keen to clone it so we could then develop aluminium-tolerant sorghum varieties more efficiently,” says Jura.

Two years after his return to Brazil,  Leon and Jura – in 2004 – submitted a joint proposal for a competitive grant for their first GCP project on aluminium tolerance in cereals, premised on AltSB. This project contributed to GCP’s foundation work on sorghum in this and other projects, the common goal being a bid to provide farmers in the developing world with sorghum crops that would be able to tolerate harsh soils. But the project contributed much more with a deep taproot in pre-history, as that which we today call ‘sorghum’, ‘maize’ and ‘rice’ were once one millions of ‘Jurassic’ years ago. More on that interesting side-story.

And since this first project, EMBRAPA and Cornell University have collaborated with several other research institutes around the world, particularly in Africa.

Left to right (foreground): Leon Kochian, Jurandir Magalhães (both EMBRAPA) and Sam Gudu (Moi University) examine crosses between Kenyan and Brazilian maize, at the Kenya Agricultural Research Institute (KARI), Kitale, in May 2010.

Left to right (foreground): Leon, Jura and Sam Gudu (Moi University) examine crosses between Kenyan and Brazilian maize, at the Kenya Agricultural Research Institute (KARI), Kitale, in May 2010.

Jura leads several EMBRAPA and GCP collaborative projects across three continents (Africa, Asia and the Americas). The partnerships forged by and through these projects go well beyond project life and frame, and will therefore continue after GCP’s sunset. Jura is both team leader and team player. And a couple of GCP projects in which Jura is part of the project team will run on in 2015 (see page 10), after GCP’s closure in December 2014.

Links

 

Jan 072015
 

Beyond chickpeas to embrace beans, chickpeas, groundnuts and pigeonpeas

Paul_w2As a scientist who comes from the dessicated drylands of the unforgiving Kerio Valley, where severe drought can mean loss of life through loss of food and animals, what comes first is food security… I could start to give something back to the community… It’s been a dream finally coming true.” – Paul Kimurto, Senior Lecturer and Professor in Crop Physiology and Breeding, Egerton University, Kenya

As a son of peasant farmers growing up in a humble home in the Rift Valley of Kenya, agriculture was, for Paul Kimurto (pictured above), not merely a vocation but a way of life: “Coming from a pastoral community, I used to take care of the cattle and other animals for my father. In my community livestock is key, as is farming of food crops such as maize, beans and finger millet.”

Covering some six kilometres each day by foot to bolster this invaluable home education with rural school, an affiliation and ever-blossoming passion for agriculture soon led him to Kenya’s Egerton University.

There, Paul excelled throughout his undergraduate course in Agricultural Sciences, and was thus hand-picked by his professors to proceed to a Master’s degree in Crop Sciences at the self-same university, before going on to obtain a German Academic Exchange Service (DAAD) scholarship to undertake a ‘sandwich’ PhD in Plant Physiology and Crop Breeding at Egerton University and the Leibniz Institute for AgriculturalEngineering (ATB) in Berlin, Germany.

… what comes first is food security… offering alternative drought-tolerant crops… is a dream finally coming true!…  GCP turned out to be one of the best and biggest relationships and collaborations we’ve had.”

Local action, global interaction
With his freshly minted PhD, Paul returned to Egerton’s faculty staff and steadily climbed the ranks to his current position as Professor and Senior Lecturer in Crop Physiology and Breeding at Egerton’s Crop Sciences Department. Yet for Paul, motivating this professional ascent throughout has been one fundamental factor:  “As a scientist who comes from a dryland area of Kerio valley, where severe drought can mean loss of food and animals, what comes first is food security,” Paul explains. “Throughout the course of my time at Egerton, as I began to understand how to develop and evaluate core crop varieties, I could start to give something back to the community, by offering alternative drought-tolerant crops like chickpeas, pigeonpeas, groundnuts and finger millet that provide farmers and their families with food security. It’s been a dream finally coming true.”

And thus one of academia’s true young-guns was forged: with an insatiable thirst for moving his discipline forward by seeking out innovative solutions to real problems on the ground, Paul focused on casting his net wide and enhancing manpower through effective collaborations, having already established fruitful working relationships with the International Maize and Wheat Improvement Center (CIMMYT), the (then) Kenya Agricultural Research Institute (KARI) and the International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) in earlier collaborative projects on dryland crops in Kenya. It was this strategy that paved the way towards teaming up with GCP, when, in 2008, Paul and his team were commissioned to lead the chickpea work in Kenya for the GCP Tropical Legumes I project (TLI), with local efforts being supported by colleagues at ICRISAT, and friends down the road at KARI undertaking the bean work of the project. Climbing aboard the GCP ship, Paul reveals, was a move worth making: “Our initial engagement with GCP started out as a small idea, but in fact, GCP turned out to be one of the best and biggest relationships and collaborations we’ve had.”

…GCP is people-oriented, and people-driven” 

Power to the people!
The success behind this happy marriage, Paul believes, is really quite simple: “The big difference with GCP is that it is people-oriented, and people-driven,” Paul observes, continuing: “GCP is building individuals: people with ideas become equipped to develop professionally.” Paul elaborates further: “I wasn’t very good at molecular breeding before, but now, my colleagues and I have been trained in molecular tools, genotyping, data management, and in the application of molecular tools in the improvement of chickpeas through GCP’s Integrated Breeding Multiyear Course. This has opened up opportunities for our local chickpea research community and beyond, which, without GCP’s support, would not have been possible for us as a developing-country institution.”

Inspecting maturity, Koibatek FTC, Bomet_R Mulwa_Sep'12_w

Inspecting pod maturity with farmers at Koibatek Farmers Training Centre in Eldama Ravine Division, Baringo County, Kenya, in September 2012. Paul is on the extreme right.

Passionate about his teaching and research work, it’s a journey of discovery Paul is excited to have shares with others: “My co-workers and PhD students have all benefitted. Technicians have been trained abroad. All my colleagues have a story to tell,” he says. And whilst these stories may range from examples of access to training, infrastructure or genomic resources, the common thread throughout is one of self-empowerment and the new-found ability to move forward as a team: “Thanks to our involvement with the GCP’s Genotyping Support Service, we now know how to send plant DNA to the some of the world’s best labs and to analyse the results, as well as to plan for the costs. With training in how to prepare the fields, and infrastructure such as irrigation systems and resources such as tablets, which help us to take data in the field more precisely, we are now generating accurate research results leading to high-quality data.”

The links we’ve established have been tremendous, and we think many of them should be long-lasting too: even without GCP

Teamwork, international connections and science with a strong sense of mission
Teaming up with other like-minded colleagues from crème de la crème institutions worldwide has also been vital, he explains: “The links we’ve established have been tremendous, and we think many of them should be long-lasting too: even without GCP, we should be able to sustain collaboration with KBioscience [now LGC Genomics] or ICRISAT for example, for genotyping or analysing our data.” He holds similar views towards GCP’s Integrated Breeding Platform (IBP): “IBP is one of the ideas which we think, even after GCP’s exit in December 2014, will continue to support our breeding programmes. My colleagues and I consult IBP regularly for a range of aspects, from markers to protocols to germplasm and the helpdesk, as well as for contacts and content available via the IBP Communities of Practice.” Paul’s colleagues are Richard Mulwa, Alice Kosgei, Serah Songok, Moses Oyier, Paul Korir, Bernard Towett, Nancy Njogu and Lilian Samoei. Paul continues: “We’ve also been encouraging our regional partners to register on IBP – I believe colleagues across Eastern and Central Africa could benefit from this one-stop shop.”

Yet whilst talking animatedly about the greater sophistication and accuracy in his work granted as a result of new infrastructure and the wealth of molecular tools and techniques now available to him and his team, at no point do Paul’s attentions stray from the all-important bigger picture of food security and sustainable livelihoods for his local community: “When we started in 2008, chickpeas were known as a minor crop, with little economic value, and in the unfavoured cluster termed ‘orphan crops’ in research. Since intensifying our work on the crop through TLI, we have gradually seen chickpeas become, thanks to their relative resilience against drought, an important rotational crop after maize and wheat during the short rains in dry highlands of Rift valley and also in the harsh environments of the Kerio Valley and swathes of Eastern Kenya.”

This GCP-funded weather station is at Koibatek Farmers Training Centre, Longisa Division, Bomet County.

This GCP-funded weather station is at Koibatek Farmers Training Centre.

Having such a back-up in place can prove a vital lifeline to farmers, Paul explains, particularly during moments of crisis, citing the 2011–2012 outbreak of the maize lethal necrosis (MLN) disease which wiped out all the maize throughout Kenya’s  Bomet County, where Paul, Richard, Bernard and their team had been working on the chickpea reference set. Those farmers who had planted chickpeas – Paul recalls Toroto and Absalom as two such fortunate souls – were food-secure. Moreover, GCP support for infrastructure such as a weather station have helped farmers in Koibatek County to predict weather patterns and anticipate rainfall, whilst an irrigation system in the area is being used by the Kenyan Ministry of Agriculture to develop improved seed varieties and pasture for farmers.

The science behind the scenes and the resultant products are of course not to be underestimated: in collaboration with ICRISAT, Paul and his team released four drought-resistant chickpea varieties in Kenya in 2012, with the self-same collaboration leading to the integration of at least four varieties of the crop using marker-assisted backcrossing, one of which is in the final stages and soon to be released for field testing. With GCP having contributed to the recent sequencing of the chickpea genome, Paul and his colleagues are now looking to up their game by possibly moving into work on biotic stresses in the crop such as diseases, an ambitious step which Paul feels confident can be realised through effective collaboration, with potential contenders for the mission including ICRISAT (for molecular markers), Ethiopia and Spain (for germplasm) and researchers at the International Center for Agricultural Research in the Dry Areas (ICARDA) for germplasm. Paul first established contact with all of these partners during GCP meetings.

By coming together, pooling skills from biotechnology, agronomy, breeding, statistics and other disciplines, we are stronger as a unit and better equipped to offer solutions to African agriculture and to the current challenges we face.”

Links that flower, a roving eye, and the heat is on!
In the meantime, the fruits of other links established since joining the GCP family are already starting to blossom. For example, TLI products such as certified seeds of chickpea varieties being released in Kenya – and in particular the yet-to-be-released marker-assisted breeding chickpea lines which are currently under evaluation – caught the eye of George Birigwa, Senior Programme Officer at the Program for Africa’s Seed Systems (PASS) initiative of the Alliance for a Green Revolution in Africa (AGRA), which is now supporting the work being undertaken by Paul and his team through the Egerton Seed Unit and Variety Development Centre (of which Paul is currently Director) at the Agro-Based Science Park.

Yet whilst Paul’s love affair with chickpeas has evidently been going from strength to strength, he has also enjoyed a healthy courtship with research in other legumes: by engaging in a Pan-African Bean Research Alliance (PABRA) bean project coordinated by the International Center for Tropical Agriculture (CIAT), Paul and his team were able to release and commercialise three bean varieties which are currently in farmers’ fields in Kenya.

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Paul (left) in the field. The crop is chickpeas of course!

With so many pots on the boil, the heat is certainly on in Paul’s research kitchen, yet he continues to navigate such daily challenges with characteristic aplomb. As a proven leader of change in his community and a ‘ can-do, make-it-happen’ kind of guy, he is driving research forward to ensure that both his school and discipline remain fresh and relevant – and he’s taking his colleagues, students and local community along with him every step of  the way.

Indeed, rallying the troops for the greater good is an achievement he values dearly: “By coming together, pooling skills from biotechnology, agronomy, breeding, statistics and other disciplines, we are stronger as a unit and better equipped to offer solutions to African agriculture and to the current challenges we face,” he affirms. This is a crusade he has no plans to abandon any time soon, as revealed when quizzed on his future aspirations and career plans: “My aim is to continue nurturing my current achievements, and to work harder to improve my abilities and provide opportunities for my institution, colleagues, students, friends and people within the region.”

With the chickpea research community thriving, resulting in concrete food-security alternatives, we raise a toast to Paul Kimurto and his chickpea champions!

Links

 

Aug 292014
 

“…I wanted to contribute in a similar way” – Eva Weltzien

 

Eva Weltzien

Eva Weltzien

Learning about the work of Nobel laureate, Norman Borlaug, in high school inspired Eva Weltzien to become a plant breeder so she too could contribute to improving the living conditions in the developing world. Today, Eva is a Principal Scientist in sorghum breeding and genetic resources at the International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) in Mali.

“Not only did Norman Borlaug revolutionise agriculture by breeding high-yielding wheat varieties, he then selflessly distributed these to the countries in the world that most needed them, saving hundreds of millions from starvation,” Eva recollects passionately, as she speaks about her scientific hero. “I remember being inspired when he won his Nobel Prize in 1970, mainly for the fact that agricultural research was actually being seen as contributing to world peace,” says Eva. “I knew then that I wanted to contribute in a similar way.”

I…wanted to take a break from… theory and instead gain an appreciation for plant breeding by working in the field”

The path to plant breeding, and pearls along the way
Eva was raised in her native Germany, as well as in Beirut, Lebanon, where she spent six years when her parents were stationed at the local university there. She credits her parents; both plant pathologists, for instilling in her a scientific mind-set from a tender age.

“They taught me to think outside the box and apply my knowledge and understanding to how I made sense of the world,” Eva recalls. “Being plant pathologists, they also encouraged me to observe the environment carefully and treat the earth with respect.”

Upon graduating from high school, Eva deferred going to university and instead worked as a seed technician for a private company in Germany. “I just wanted to take a break from studying theory and instead gain an appreciation for plant breeding by working in the field,” says Eva.

After one year with the company, Eva was ready to start university. During the decade that followed, she completed a Diploma in Agricultural Biology (University of Hohenheim, 1981) and a PhD in Agriculture (Munich University, 1986).

A year after completing her PhD, Eva accepted a postdoc position at Iowa State University, USA, where she met her future husband Fred Rattunde. After a few years, both Eva and Fred moved to India to work with ICRISAT. “I’ve been working for ICRISAT for almost 27 years now,” says Eva. “When I first started, I was working in pearl millet breeding.”

The key challenges have been improving the infrastructure of the national research facilities… as well as increasing the technical training for local researchers…this has slowly improved, particularly in the last four years with the funding and help through the GCP Sorghum Research Initiative.…we can see our work making an impact on people’s lives…”

Off to Africa, and bearing fruit
In 1998, ICRISAT offered Eva and Fred positions in Mali where they would take responsibility for the Institute’s sorghum-breeding programme in West Africa.

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Evaluating Eva: In Dioila district, Mali, evaluating the panicles of a new sorghum line after harvest.

“It was a great challenge that we both wanted to explore,” says Eva. “The key challenges have been improving the infrastructure of the national research facilities to do the research as well as increasing the technical training for local agronomists and researchers. Over the past 15 years, this has slowly improved, particularly in the last four years, with the funding and facilitation through the GCP Sorghum Research Initiative. Now we can see our work making an impact on people’s lives in West Africa.” (see GCP’s work on infrastructure improvement)

…we are closer to delivering more robust sorghum varieties which will help farmers and feed the ever-growing population in West Africa.”

Improving drought tolerance in sorghum for Africa
The second phase of GCP’s Sorghum Research Initiative focuses on Mali, where sorghum-growing areas are large, and distributed over a wide range of rainfall regimes.

Eva and her team are currently collaborating with local researchers at L’Institut d’économie rurale (IER), Mali and France’s Centre de coopération internationale en recherche agronomique pour le développement (CIRAD) on a project to test a novel molecular-breeding approach – backcross nested association mapping (BCNAM). Eva says the approach has the potential to halve the time it takes to develop local sorghum varieties with improved yield and adaptability to poor soil fertility conditions.

“We don’t have these type of molecular-breeding resources available in Mali, so it’s really exciting to be a part of this project.”  Still, Eva and her colleagues continue to press forwards in this new frontier in plant science, making good advances in another parallel but closely related project that Eva leads in the GCP Comparative Genomics Research Initiative.

Eva continues, “We’ve had good results in terms of field trials, despite the political situation. Overall, we feel the experience is enhancing our capacity here in Mali, and that we are closer to delivering more robust sorghum varieties which will help farmers and feed the ever-growing population in West Africa.”

Slides (with more links after the slides)

Links

Aug 152014
 

 

Samuel Gudu

Samuel Gudu

Having funding to support PhD students and provide them with the resources they need to complete their research is very fulfilling and will go a long way to enhance the long-term success of our goal: to provide Kenyan farmers with cereal varieties that will improve their yields and make their livelihood more secure and sustainable.” – Samuel Gudu, Professor and Deputy Vice-Chancellor (Planning & Development) at Moi University, and now Principal, Rongo University College: a Constituent College of Moi University, Kenya.

Growing up, and getting dirty
Learner, teacher and leader. Sam Gudu has been all these, but this doesn’t mean he doesn’t like to get his hands dirty.

Growing up in a small fishing village on the banks of Lake Victoria, in Western Kenya, Sam was always helping his parents to fish and garden, or his grandparents to muster cattle.

“I remember spending long hours before and after school either on the lake or in the field helping to catch, harvest and produce enough food to eat and support our family,” reminisces Sam.

He attributes this “hard and honest” work to why he still enjoys being in the field.

“Even though I now spend most of my days doing administration work, I’m always trying to get out into the field to get my hands dirty and see how our research is helping to make the lives of Kenyan farmers a lot more profitable and sustainable,” he says.

Sam in a maize field in Kenya.

Doing what he likes to do best: Sam in a maize field in Kenya.

I was… captivated by the study of genetics as it focused on what controlled life.”

Taking control: bonded to genetics, at home and away
Sam says his love for the land transferred to an interest and then passion in the classroom during high school. “I became very interested in Biology as I wanted to know how nature worked,” says Sam. “I was particularly captivated by the study of genetics as it focused on what controlled life.”

This interest grew during his undergraduate years at the University of Nairobi where he completed a Bachelor of Science in Agriculture and a Master’s of Science in Agriculture, focusing on genetics and plant breeding.

“I fondly remember a lecturer during my master’s degree studies who would continually give us challenges to test in the field and in the lab. If you had a viable idea he supported you to design an experiment to test your theory. I like to use the same method in teaching my students. I discuss quite a lot with my students and I encourage them to disagree if they use scientific process.”

Driven by an ever-growing passion and enthusiasm, Sam secured a scholarship to travel to Canada to undertake a PhD in Plant Genetics and Biotechnology at the University of Guelph.

[There has been an] influx of young Kenyans who are choosing degrees in science. The Kenyan Government has recently increased its funding for science and research…”

Nurturing the next breed of geneticists
After graduating from Guelph in 1993, Sam returned to Kenya to lecture at Moi University where he initiated and helped expand teaching and research in the disciplines of Genetic Engineering, Biotechnology and Molecular Biology.

In the past two decades, he has recruited young talented graduates in genetics and helped acquire advanced laboratory equipment that has enabled practical teaching and research in molecular biology.

“I wouldn’t be where I am now were it not for all the assistance I received from my teachers, lecturers and supervisors; notably my PhD supervisor – Prof Ken Kasha of the University of Guelph. So I’ve always tried my best to give the same assistance to my students. It’s been hard work but very rewarding, especially when you see your students graduate to become peers and colleagues.” (Meet some of Sam’s students)

Sam (2nd right), with some of his young charges: Thomas Matonyei (far left) , Edward Saina (2nd left) and Evans Ouma (far right)

Sam (2nd right), with some of his young charges: Thomas Matonyei (far left), Edward Saina (2nd left) and Evans Ouma (far right).

Sam is particularly buoyed by the influx of young Kenyans who are choosing degrees in science.

“The Kenyan Government has recently increased its funding for science and research to two percent of GDP,” explains Sam. “This has not only helped us compete in the world of research but has helped raise the profile of science as a career.”

Knowing which genes are responsible for aluminium tolerance will allow us to more precisely select for aluminium tolerance in our breeding programmes, reducing the time it takes for us to breed varieties that will have improved yields in acidic soils without the use of costly inputs such as lime or fertiliser.” (See the work that Sam does in this area with other partners outside Kenya)

So far we have produced 10 inbred lines that are outstanding for phosphorus efficiency, and two that were outstanding for aluminium toxicity. We are now testing unique verities developed for acid soils of Kenya.”

Slashing costs, increasing yields and resilience: genes to the rescue
Currently, Sam and his team of young researchers at Moi University are working with several other research facilities around the world (Brazilian Agricultural Research Corporation, EMBRAPA; Cornell University, USA; the International Rice Research Institute (IRRI); Japan’s International Research Center for Agricultural Sciences, JIRCAS; and the Kenya Agricultural Research Institute, KARI–Kitale) to develop high-yielding maize varieties adapted to acid soils in East Africa, using molecular and conventional breeding approaches.

Can you spot Sam? It’s a dual life. Here, he sheds his field clothes in this 2011 suit-and-tie moment with Moi University and other colleagues involved in the projects he leads. Left to right: P Kisinyo, J Agalo, V Mugalavai, B Were, D Ligeyo, S Gudu, R Okalebo and A Onkware.

Acid soils cover almost 13 per cent of arable land in Kenya, and most of the maize-growing areas in Kenya. In most of these areas, maize yields are reduced by almost 60 per cent. Aluminium toxicity is partly responsible for the low and declining yields.

“We found that most local maize varieties and landraces grown in acid soils are sensitive to aluminium toxicity. The aluminium reduces root growth and as such the plant cannot efficiently tap into native soil phosphorus, or even added phosphorus fertiliser. However, there are some varieties of maize that are suited to the conditions even if you don’t use lime to improve the soil’s pH. So far we have produced 10 inbred lines that are outstanding for phosphorus efficiency, and two that were outstanding for aluminium toxicity. We are now testing unique varieties developed for acid soils of Kenya.”

Sam (left)   a group of farmers and alking to farmers and researchers at Sega, Western Kenya, in June 2009

Sam (left) addressing a mixed group of farmers and researchers at Sega, Western Kenya, in June 2009.

In a related project, Sam is working with the same partners to understand the molecular and genetic basis for aluminium tolerance.

“Knowing which genes are responsible for aluminium tolerance will allow us to more precisely select for aluminium tolerance in our breeding programmes, reducing the time it takes for us to breed varieties that will have improved yields in acidic soils without the use of costly inputs such as lime or fertiliser.”

 … my greatest achievements thus far have been those which have benefited farmers and my students.”

 Summing up success
For Sam, the greatest two successes in his career have not been personal.

“If I’m honest, I have to say my greatest achievements thus far have been those which have benefited farmers and my students. Having funding to support PhD students and provide them with the resources they need to complete their research is very fulfilling and will go a long way to enhance the long-term success of our goal: to provide Kenyan farmers with cereal varieties that will improve their yields and make their livelihoods more secure and sustainable.”

With a dozen aluminium-tolerant and phosphorus-efficient breeding lines under their belt already, and two lines submitted for National Variety Trials (a pre-requisite step to registration and release to farmers), Sam and his team seem well on their way towards their goal, and we wish them well in their quest and labour.

Links:

 

Jun 242014
 

Triumphs and tragedies, pitfalls and potential of the ‘camel crop’Cassava leaf. Photo: N Palmer/CIAT

We travel through space and time, with a pair of researchers who have a pronounced passion for a plant brought to Africa by seafaring Portuguese traders in the 16th century. Fastforwarding to today, half a millennium later, the plant is widespread and deep inland, and is the staple food for Africa’s most populous nation – Nigeria.

Meet cassava, the survivor. After rice and maize, cassava is the third-largest source of carbohydrate in the tropics. Surviving, nay thriving, in poor soils and shaking off the vagaries of weather – including an exceptionally high threshold for drought – little wonder that cassava, the ‘camel’ of crops is naturally the main staple in Nigeria. And with that, it has propelled Nigeria to the very top of the cassava totem pole as the world’s leading cassava producer, and consumer: most Nigerians eat cassava in one form or another practically every day.

Great, huh? But there’s also a darker side to cassava, as we will soon find out from our two cassava experts. For starters, the undisputed global cassava giant, Nigeria, produces just enough to feed herself. Even if there were a surplus for the external demand, farming families, which make up 70 percent of the Nigerian population, have limited access to these lucrative external markets. Secondly, cassava mosaic disease (CMD) and cassava brown streak disease (CBSD) are deadly in Africa. Plus, cassava is a late bloomer (up to two years growth cycle, typically one year), so breeding and testing improved varieties takes time. Finally, cassava is most definitely not à la mode at all in modern crop breeding: the crop is an unfashionably late entrant into the world of molecular breeding, owing to its complex genetics which denied cassava the molecular tools that open the door to this glamour world of ‘crop supermodels’.

Emmanuel Okogbenin (left) and Chiedozie Egesi (right) in  a cassava field.

Emmanuel Okogbenin (left) and Chiedozie Egesi (right) in a cassava field.

But all is not doom and gloom, which inexorably dissolve in the face of dogged determination. All the above notwithstanding, cassava’s green revolution seems to be decidedly on the way in Nigeria, ably led by born-and-bred sons of the soil: Chiedozie Egesi and Emmanuel Okogbenin (pictured right) are plant breeders and geneticists at the National Root Crops Research Institute (NRCRI). With 36 years’ collective cassava research experience between them, the two men are passionate about getting the best out of Nigeria’s main staple crop, and getting their hands into the sod while about it: “I’m a plant breeder,” says Chiedozie, with pride. “I don’t just work in a laboratory. I am also in the field to experience the realities.”

Hitting two birds with one stone…two stones are even better!
As Principal Investigators (PIs) leading three different projects in the GCP-funded Cassava Research Initiative, Chiedozie and Emmanuel, together with other colleagues from across Africa, form a formidable team. They also share a vision to enable farmers increase cassava production for cash, beyond subsistence. This means ensuring farmers have new varieties of cassava that guarantee high starch-rich yields in the face of evolving diseases and capricious weather.

Chiedozie is one of cassava’s biggest fans. His affection for, and connection to, cassava is almost personal and definitely paternal. He is determined to deploy the best plant-breeding techniques to not only enhance cassava’s commercial value, but to also protect the crop against future disease outbreaks, including ‘defensive‘ breading. But more on that later…

Emmanuel is equally committed to the cassava cause. As part of his brief, Emmanuel liaises with the Nigerian government, to develop for – and promote to – farmers high-starch cassava varieties. This ensures a carefully crafted multi-pronged strategy to revolutionise cassava: NRCRI develops and releases improved varieties, buttressed by financial incentives and marketing opportunities that encourage farmers to grow and sell more cassava, which spurs production, thereby simultaneously boosting food security while also improving livelihoods.

erect cass1_LS 4 web

Standing tall. Disease resistance and high starch and yield aside, farmers also prefer an upright architecture, which not only significantly increases the number of plants per unit, but also favours intercropping, a perennial favourite   for cassava farmers.

Cross-continental crosses and cousins, magic for making time, and clocking a first for cassava

No one has been able to manufacture time yet, so how can breeders get around cassava’s notoriously long breeding cycle? MAS (marker-assisted selection) is crop breeding’s magic key for making time. And just as humans can benefit from healthy donor organ replacement, so too does cassava, with cross-continental cousins donating genes to rescue the cousin in need. Latin American cassava is nutrient-rich, while African cassava is hardier, being more resilient to pests, disease and harsh environments.

Thanks to marker-assisted breeding, CMD resistance from African cassava can now be rapidly ‘injected’ much faster into Latin American cassava for release in Africa. Consequently, in just a three-year span (2010–2012), Chiedozie, Emmanuel, Martin Fregene of the Donald Danforth Plant Science Center (USA) and the NRCRI team, released two new cassava varieties from Latin American genetic backgrounds (CR41-10 and CR36-5). These varieties, developed with GCP funding, are the first molecular-bred cassava ever to be released, meaning they are a momentous milestone in cassava’s belated but steady march towards its own green revolution.

Marker-assisted selection is much cheaper, and more focused.” 

On the cusp of a collaborative cassava revolution: on your marks…
With GCP funding, Chiedozie and Emmanuel have been able to use the latest molecular-breeding techniques to speed up CMD resistance. Using marker-assisted selection (MAS) which is much more efficient, the scientists identified plants combining CMD resistance with desirable genetic traits.

“MAS for CMD resistance from Latin American germplasm is much cheaper, and more focused,” explains Emmanuel. “There is no longer any need to ship in tonnes of plant material to Africa. We can narrow down our search at an early stage by selecting only material that displays markers for the genetic traits we’re looking for.” Using markers, combining traits (known as ‘gene pyramiding’) for CMD resistance is faster and more efficient, as it is difficult to distinguish phenotypes with multiple resistance in the field by just observing with the naked eye. This is what makes marker-assisted breeding so effective and desirable in Africa.

GCP’s mode of doing business coupled with its community spirit has spurred the NRCRI scientists to cast their eyes further out to the wider horizon beyond their own borders.

By collaborating with research centres in other parts of the world, Emmanuel and Chiedozie have made remarkable strides in cassava breeding. According to Emmanuel, “GCP helped us make links with advanced laboratories and service providers like LGC Genomics. The outsourcing of genotyping activities for molecular breeding initiatives is very significant, as it enables us to carry out analyses not otherwise possible.”

We can’t afford to sit idle until it comes – we need to be armed and on the ready.”

‘Defensive’ breeding: partnerships to pre-empt catastrophe and combat disease
Closer home in Africa, as PI of the corollary African breeders community of practice (CoP) project, Emmanuel co-organises regular workshops with plant breeders from a dozen other countries (Côte d’Ivoire, DR Congo, Ethiopia, Ghana, Kenya,  Liberia, Malawi, Mozambique, Sierra Leone, Tanzania, Uganda and South Sudan). These events are an opportunity to share knowledge on molecular breeding and compare notes.

Of the diseases that afflict cassava, CBSD is the most devastating. Mercifully, in Nigeria, the disease is non-existent, but Chiedozie is emphatic that this is by no means cause for complacency. “If CBSD gets to Nigeria, it would be a monumental catastrophe!” he cautions. “We can’t afford to sit idle until it comes – we need to be armed and on the ready.”

Putting words to action, though this work on CBSD resistance is still in its early stages, more than 1,000 cassava genotypes (different genetic combinations) have already have been screened in the course of just one year. Chiedozie hopes that the team will be able to identify key genetic markers, and validate these in field trials in Tanzania, where CBSD is widespread. This East African stopover, Chiedozie emphasises, is a crucial checkpoint in the West African process. So the cassava CoP not only provides moral but also material support.

And Africa is not the limit. GCP-funded work on CMD resistance is more advanced than the CBSD work, though the real breakthrough in CMD only happened recently, on the international arena within which the African breeders now operate. According to Chiedozie, two entire decades of screening cassava genotypes from Latin America yielded no resistance to CMD. The reason for this is that although it is widespread in Africa, CMD is non-existent in Latin America.

Through international collaborative efforts, cassava scientists, led by Martin Fregene (now based in USA), screened plants from Nigeria and discovered markers for the CMD2 gene, indicating resistance to CMD. Once they had found these markers, the scientists were off and away! By taking the best of the Latin American material and crossing it with Nigerian genotypes that have CMD resistance, promising lines were developed from which the Nigerian team produced two new varieties. These varieties, CR41-10 and CR36-5, have already been released to farmers, and that is not all. More varieties bred using these two as parents are in the pipeline.

“GCP funding has given us the opportunity to show that a national organisation can do the job and deliver.” 

 

Delivery attracts
The success of the CGP-funded cassava research in Nigeria lies in its in-country leadership. Chiedozie, Emmanuel and Martin are native Nigerian scientists and as such are – in many ways – best placed to drive a research collaboration to benefit the country’s farmers and boost food security. “GCP funding has given us the opportunity to show that a national organisation can do the job and deliver,” says Chiedozie.

This proven expertise has helped NRCRI forge other partnerships and attract more financial support, for example from the Bill & Melinda Gates Foundation for a project on genomic selection. GCP support has also bolstered communications with the Nigerian government, which has launched financial instruments, such as a wheat tariff,* to boost cassava production and use.

[Editors note: * wheat tariff: The Nigerian government is trying to reduce wheat import bills and also boost cassava commercialisation by promoting 20 percent wheat substitution in bread-making. Tariffs are being imposed on wheat to dissuade heavy imports and encourage utilisation of high-quality cassava flour for bread.]

“The government feels that to quickly change the fortunes of farmers, cassava is the way to go,” explains Emmanuel. He clarifies, “The tariff from wheat is expected to be ploughed back to support agricultural development – especially the cassava sector – as the government seeks to increase cassava production to support flour mills. Cassava offers a huge opportunity to transform the agricultural economy and stimulate rural development, including rapid creation of employment for youth.”

The Nigerian government is right in step aiding cassava’s march towards the crop’s own green revolution, as is evident in the the Minister of Agriculture’s tweet earlier this year, and in his video interview below. See also related media story, ‘Long wait for cassava bread’.

Clearly, the ‘camel’ crop – once considered an ‘orphan’ in research  –  has travelled as far in science as in geography, and it is a precious asset to deploy for food production in a climate-change-prone world. As Emmanuel observes, cassava’s future can only be brighter!

Slides by Chiedozie and Emmanuel

 

More links

 

May 302014
 
Rogério Chiulele

Rogério Chiulele

 

Today, we travel the Milky Way on a voyage to Mozambique. Our man along the Milky Way is Rogério Marcos Chiulele (pictured), a lecturer at Mozambique’s Universidade Eduardo Mondlane’s Crop Science Department. He is also the lead scientist for cowpea research in Mozambique for the Tropical Legumes I (TLI) project. This gives Rogério a crucial tri-focal down-to-earth and away-from-the-clouds perspective on cowpea pedagogy, research and development. It is through this pragmatic triple-lens prism that Rogerio speaks to us today, once he’s captained us safely back from the stars to Planet Earth, Southeast Africa. After the protein and profit, next stop for him and team is ridding cowpeas of pod-sucking pests, among other things slated for the future. But back from the future to the present and its rooted realities…Problems, yes, but also lots of good scores, plus a deft sleight of hand that are bound to have you starry-eyed, we bet.

…cowpeas rank fourth as the most cultivated crop…”

Q: Tell us about Mozambique and cowpeas: are they important?

The devastating effects of nematodes on cowpea roots.

The devastating effects of nematodes on cowpea roots.

In Mozambique, cowpeas are an important source of food, for both protein and profit, particularly for the resource-poor households that benefit from cowpea income and nutrition. In terms of cultivation, cowpeas rank fourth as the most cultivated crop after maize, cassava and groundnuts, accounting for about 9 percent of the total cultivated area, and estimated at nearly four million hectares of smallholder farms. The crop is produced for grain and leaves, mostly for household consumption but it is becoming increasingly important as a supplement for household income.

But while its potential for food, protein and income is recognised, the realisation of such potential is still limited by drought due to irregular and insufficient rain; affliction by pests such as aphids, flower thrips and nematodes; diseases such as cowpea aphid mosaic virus and cowpea golden mosaic virus; and cultivation of low-yielding and non-improved varieties.

…we backcross to varieties with traits that farmers prefer…”

Q: And on cowpea research and breeding?
Since 2008, Universidade Eduardo Mondlane [UEM] established a cowpea-breeding programme for addressing some of the limiting constraints affecting cowpea production and productivity. This has been possible through collaboration with different funding institutions such as the Generation Challenge Programme.

Photo: UEM

2008: Screening of the 300 genotypes.

That same year [2008], a UEM research team that I coordinate qualified for a GCP capacity-building à la carte grant. In this project, we screened 300 Mozambican cowpea lines for drought tolerance. From these, we identified 84 genotypes that were either high-yielding or drought-tolerant. We further evaluated the 84 genotypes for another three seasons in two locations. From the 84, we identified six genotypes that not only had the two sought-after traits, but were also adapted to different environments.

In 2010, the UEM team joined the TLI project. For the six pre-identified genotypes, the UEM breeding programme is using marker-assisted recurrent selection [MARS] and marker-assisted backcrossing [MABC], combining drought tolerance and resistance to major biotic stresses occurring in Mozambique. In MABC, we are conducting a backcross to varieties with traits that farmers prefer, which includes aspects such as large seeds, early maturity and high leaf production.

…we conducted a farmers’ participatory varietal selection to glean farmers’ perceptions and preferences on cowpea varieties and traits…”

Q: What is the main focus in your work, and how and when do farmers come in?
The breeding work conducted by UEM is targeting all Mozambican agroecologies, but with particular focus on southern Mozambique which is drought-prone. In addition to drought, the area is plagued by many pests such as aphids, flower thrips, nematodes and pod-sucking pests. So, in addition to drought tolerance, we are conducting screening and selection for resistance to aphids, flower thrips and nematodes. In the near future, we will start screening for resistance to pod-sucking pests.

2009: field screening of the 84 genotypes in diff locations.

2009: Rogério during field screening of the 84 genotypes in different locations.

In 2009, we conducted a farmers’ participatory varietal selection to glean farmers’ perceptions and preferences on cowpea varieties and traits. From the study, six of the lines passed participatory variety selection with farmers, as they were large-seeded with good leaf production which provides additional food.

we hope to release three varieties in 2015…Our involvement with GCP has not only increased our exposure, but also brought along tangible benefits… I firmly believe black-eyed peas can really make a difference.”

Q: To what would you attribute the successes your team is scoring, and what are your goals for the future, besides screening for pod-sucking pests?
The success of the work that the Eduardo Mondlane team is doing is partly due to the collaboration and partnership with USA’s University of California, Riverside [UCR]. UCR sent us 60 lines from the GCP cowpea reference set* [Editorial note: see explanation at the bottom], which we evaluated for drought tolerance for four seasons in two locations – one with average rainfall and the other drought-prone. As these lines were already drought-tolerant, we tested them for adaptation to the local environment, and for high yield. From the set, we hope to release three varieties in 2015. In addition, for evaluating the different varieties, we also crossed the local varieties with black-eyed peas, which have a huge market appeal: local varieties fetch roughly half a US dollar per kilo, compared to black-eyed peas whose price is in the region of four to five US dollars.

2013: multilocation trials.

2013: multilocation trials.

Our involvement with GCP has not only increased our exposure, but also brought along tangible benefits. For example, previously, nothing was being done on drought tolerance for cowpeas. But now we receive and exchange material, for example, the black-eyed peas from UCR that we received through GCP, which are set to boost production and markets, thereby improving lives and livelihoods. Amongst the varieties we are proposing to release is one black-eye type. I firmly believe black-eyed peas can really make a difference.

In addition, besides funding a PhD for one of our researchers, Arsenio Ndeve, who is currently at UCR, the Generation Challenge Programme, contributed to improvement on storage and irrigation facilities. We purchased five deep freezers for seed storage and one irrigation pump. Presently, we have adequate storage facilities and we conduct trials even during the off-season, thanks to the irrigation pump provided by GCP.

****

And on that upbeat note even as the challenge ahead is immense, today’s chat with Rogério ends here. To both pod-sucking pests and all manner of plagues on cowpeas, beware, as thy days are numbered: it would seem that Rogério and team firmly say: “A pox on both your houses!”

*A ‘reference set’ is a sub-sample of existing germplasm collections that facilitates and enables access to existing crop diversity for desired traits, such as drought tolerance or resistance to disease or pests

Links

May 122014
 

 

Omari Mponda

Omari Mponda

After getting a good grounding on the realities of groundnut research from Vincent, our next stop is East Africa, Tanzania, where we meet Omari Mponda (pictured). Omari is a Principal Agricultural Officer and plant breeder at Tanzania’s Agricultural Research Institute (ARI), Naliendele, and country groundnut research leader for the Tropical Legumes I (TLI) project, implemented through our Legumes Research Initiative.  Groundnut production in Tanzania is hampered by drought in the central region and by rosette and other foliar diseases in all regions. But all is not bleak, and there is a ray of hope: “We’ve been able to identify good groundnut-breeding material for Tanzania for both drought tolerance as well as disease resistance,” says Omari. Omari’s team are also now carrying their own crosses, and happy about it. Read on to find out why they are not labouring under the weight of the crosses they carry…

…we have already released five varieties…TLI’s major investment in Tanzania’s groundnut breeding has been the irrigation system… Frankly, we were not used to being so well-equipped!”

Q: How  did you go about identifying appropriate groundnut-breeding material for Tanzania?
A: We received 300 reference-set lines from ICRISAT [International Crops Research Institute for the Semi-Arid Tropics], which we then genotyped over three years [2008– 2010] for both drought tolerance and disease resistance. After we identified the best varieties, these were advanced to TLII [TLI’s sister project] for participatory variety selection with farmers in 2011–2012, followed by seed multiplication. From our work with ICRISAT, we have already released five varieties.

Harvesting ref set collection at Naliendele_w

Harvesting the groundnut reference-set collection at Naliendele. A ‘reference set’ is a sub-sample of existing germplasm collections that facilitates and enables access to existing crop diversity for desired traits, such as drought tolerance or resistance to disease or pests.

ARI–Naliendele has also benefitted from both human and infrastructure capacity building. Our scientists and technicians were trained in drought phenotyping at ICRISAT Headquarters in India. One of our research assistants, Mashamba Philipo, benefitted from six-month training, following which he advanced to an MSc specialising in drought phenotpying using molecular breeding. In his work, he is now using drought germplasm received from ICRISAT. In terms of laboratory and field infrastructure, the station got irrigation equipment to optimise drought-phenotyping trials. Precision phenotyping and accurate phenotypic data are indispensable for effective molecular breeding. To facilitate this, ARI–Naliendele benefitted from computers, measuring scales, laboratory ware and a portable weather station, all in a bid to assure good information on phenotyping. But by far, TLI’s major investment in Tanzania’s groundnut breeding has been the irrigation system which is about to be completed. This will be very useful as we enter TLIII for drought phenotyping.

 

For us, this is a big achievement to be able to do national crosses. Previously, we relied on ICRISAT…we are advancing to a functional breeding programme in Tanzania… gains made are not only sustainable, but also give us independence and autonomy to operate..We developing-country scientists are used to applied research and conventional breeding, but we now see the value and the need for adjusting ourselves to understand the use of molecular markers in groundnut breeding.”

Omari (right), with Hannibal Muhtar (left), who was contracted by GCP to implement infrastructure improvement for ARI Naliendele. See http://bit.ly/1hriGRp

Flashback to 2010: Omari (right), with Hannibal Muhtar (left), who was contracted by GCP to implement infrastructure improvement for ARI Naliendele, and other institutes. See http://bit.ly/1hriGRp

Q: What difference has participating in TLI made?
A: Frankly, we were not used to being so well-equipped, neither with dealing with such a large volume as 300 lines! But we filtered down and selected the well-performing lines which had the desired traits, and we built on these good lines. The equipment purchased through the project not only helped us with the actual phenotyping and being able to accurately confirm selected lines, but also made it possible for us to conduct off-season trials.

We’re learning hybridisation skills so that we can use TLI donors to improve local varieties, and our technicians have been specifically trained in this area. For us, this is a big achievement to be able to do national crosses. Previously, we relied on ICRISAT doing the crosses for us, but we can now do our own crosses. The difference this makes is that we are advancing to a functional breeding programme in Tanzania, meaning the gains made are not only sustainable, but also give us independence and autonomy to operate. Consequently, we are coming up with other segregating material from what we’ve already obtained, depending on the trait of interest we are after.

Another big benefit is directly interacting with world-class scientists in the international arena through the GCP community and connections – top-rated experts not just from ICRISAT, but also from IITA, CIAT, EMBRAPA [Brazil], and China’s DNA Research Institute. We have learnt a lot from them, especially during our annual review meetings. We developing-country scientists are used to applied research and conventional breeding, but we now see the value and the need for adjusting ourselves to understand the use of molecular markers in groundnut breeding. We now look forward to TLIII where we expect to make impact by practically applying our knowledge to groundnut production in Tanzania.

Interesting! And this gets us squarely back to capacity building. What are your goals or aspirations in this area?
A: Let us not forget that TLI is implemented by the national programmes. In Africa, capacity building is critical, and people want to be trained. I would love to see fulltime scientists advance to PhD level in these areas which are a new way of doing business for us. I would love for us to have the capacity to adapt to our own environment for QTLs [quantitative trait loci], QTL mapping, and marker-assisted selection. Such capacity at national level would be very welcome. We also hope to link with advanced labs such as BecA [Biosciences eastern and southern Africa] for TLI activities, and to go beyond service provision with them so that our scientists can go to these labs and learn.

There should also be exchange visits between scientists for learning and sharing, to get up to date on the latest methods and technologies out there. For GCP’s Integrated Breeding Platform [IBP], this would help IBP developers to design reality-based tools, and also to benefit from user input in refining the tools.

Links

SLIDES by Omari on groundnut research and research data management in Tanzania

 

Apr 042014
 

 

Phil Roberts

Phil Roberts

Like its legume relatives, cowpeas belong to a cluster of crops that are still referred to in some spheres of the crop-breeding world as ‘orphan crops’. This, because they have largely been bypassed by the unprecedented advances that have propelled ‘bigger’ crops into the world of molecular breeding, endowed as they are with the genomic resources necessary. But as we shall hear from Phil Roberts (pictured), of the University of California–Riverside, USA, and also the cowpea research leader for the Tropical Legumes I Project (TLI), despite the prefix in the  name, this ‘little kid’ in the ‘breeding block’ called cowpeas is uncowed and unbowed, confidently striding into the world of modern crop breeding, right alongside the ‘big boys’! What more on this new kid on the block of modern molecular breeding? Phil’s at hand to fill us in…

Vigna the VIP that shrinks with the violets
But is no shrinking violet, by any means, as we shall see. Also known  as niébé in francophone Africa, and in USA as black-eyed peas (no relation to the musical group, however, hence no capitals!), this drought-tolerant ancient crop (Vigna unguiculata [L] Walp) originated in West Africa. It is highly efficient in fixing nitrogen in the unforgiving and dry sandy soils of the drier tropics. And that is not all. This modest VIP is not addicted to the limelight and is in fact outright lowly and ultra-social: like their fetching African counterpart in the flower family, the African violet, cowpeas will contentedly thrive under the canopy of others, blooming in the shade and growing alongside various cereal and root crops, without going suicidal for lack of limelight and being in the crowd. With such an easy-going personality, added to their adaptability, cowpeas have sprinted ahead to become the most important grain legume in sub-Saharan Africa for both subsistence and cash. But – as always – there are two sides to every story, and sadly, not all about cowpeas is stellar…

Improved varieties are urgently needed to narrow the gap between actual and potential yields… modern breeding techniques… can play a vital role”

A cowpea experimental plot at IITA.

A cowpea experimental plot at IITA.

What could be, and what molecular breeding has to do with it
Yields are low, only reaching a mere 10 to 30 percent of their potential, primarily because of insect- and disease-attack, sometimes further compounded by chronic drought in the desiccated drylands cowpeas generally call home. “Improved varieties are urgently needed to narrow the gap between actual and potential yields,” says Phil. The cowpea project he leads in TLI is implemented through GCP’s Legume Research Initiative. Phil adds, “Such varieties are particularly valuable on small farms, where costly agricultural inputs are not an option. Modern breeding techniques, resulting from the genomics revolution, can play a vital role in improving cowpea materials.”

He and his research team are therefore developing genomic resources that country-based breeding programmes can use. Target-country partners are Institut de l’Environnement et de Recherches Agricoles (INERA) in Burkina Faso; Universidade Eduardo Mondlane in Mozambique; and Institut Sénégalais de Recherches Agricoles (ISRA) in Senegal. Other partners are the International Institute of Tropical Agriculture (IITA) headquartered in Nigeria and USA’s Feed the Future Innovation Labs for Collaborative Research on Grain Legumes and for Climate Resilient Cowpeas.

It’s a lot easier and quicker, and certainly less hit-or-miss than traditional methods!… By eliminating some phenotyping steps and identifying plants carrying positive-trait alleles for use in crossing, they will also shorten the time needed to breed better-adapted cowpea varieties preferred by farmers and markets.”

Cowpea seller at Bodija Market, Ibadan, Nigeria.

Cowpea seller at Bodija Market, Ibadan, Nigeria.

 

On target, and multiplying the score
[First, a rapid lesson on plant-genetics jargon so we can continue our story uninterrupted: ‘QTLs’ stands for quantitative trait loci, a technical term in quantitative genetics to describe the locations where genetic variation is associated with variation in a quantitative trait. QTL analysis estimates how many genes control a particular trait. ‘Allele’ means an alternative form of a the same gene. Continuing with the story…]

The curved shape means that these cowpea pods are mature and ready for harvesting.

Culinary curves and curls: the curved shape means that these cowpea pods are mature and ripe for harvesting.

“We first verified 30 cowpea lines as sources of drought tolerance and pest resistance,” Phil recalls. “Using molecular markers, we can identify the genomic regions of the QTLs that are responsible for the desired target phenotype, and stack those QTLs to improve germplasm resistance to drought or pests. It’s a lot easier and quicker, and certainly less hit-or-miss than traditional methods! However, standing alone, QTLs are not the silver bullet in plant breeding. What happens is that QTL information complements visual selection. Moreover, QTL discovery must be based on accurate phenotyping information, which is the starting point, providing pointers on where to look within the cowpea genome. Molecular breeding can improve varieties for several traits in tandem,” suggests Phil. “Hence, farmers can expect a more rapid delivery of cowpea varieties that are not only higher-yielding, but also resistant to several stresses at once.”

And what are Phil and team doing to contribute to making this happen?

The genomic resources from Phase I – especially genotyping platforms and QTL knowledge – are being used in Phase II of the TLI Project to establish breeding paradigms, using molecular breeding approaches,” Phil reveals. He adds that these approaches include marker-assisted recurrent selection (MARS) and marker assisted back-crossing (MABC). “These paradigms were tested in the cowpea target countries in Africa,” Phil continues. “By eliminating some phenotyping steps and identifying plants carrying positive-trait alleles for use in crossing, they will also shorten the time needed to breed better-adapted cowpea varieties preferred by farmers and markets.”

… best-yielding lines will be released as improved varieties… others will be used…as elite parents…”

Future work
What of the future? Phil fills us in: “The advanced breeding lines developed in TLI Phase II are now entering multi-location performance testing in the target African countries. It is expected that best-yielding lines will be released as improved varieties, while others will be used in the breeding programmes as elite parents for generating new breeding lines for cowpeas.”

Clearly then, the job is not yet done, as the ultimate goal is to deliver better cowpeas to farmers. But while this goal is yet to be attained and – realistically – can only be some more years down the road, it is also equally clear that Phil and his team have already chalked up remarkable achievements in the quest to improve cowpeas. They hope to continue pressing onwards and upwards in the proposed Tropical Legumes III Project, the anticipated successor to TLI and its twin project TLII – Tropical Legumes II.

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Mar 042014
 
‘Made (up) in Ghana’

In the world of crop research as in the fashion industry, there are super-models, mere models, spectators and rank outsiders. Make no bones about it, trusty old cassava (Manihot esculenta) is a crop of very modest beginnings, but now finally strutting the research catwalk alongside the biggest and the best.

Elizabeth Parkes

Elizabeth Parkes

An ancient crop thought to have been first domesticated in Latin America more than 10,000 years ago, it was exported by Portuguese slave traders from Brazil to Africa in the 16th century as a cheap source of carbohydrates. From there, today we travel half a millennium forward in time – and in space, on to Ghana – to catch up with the latest on cassava in the 21st century.

Come on a guided tour with Elizabeth Parkes (pictured), of Ghana’s Crops Research Institute (CRI, of the Council for Scientific and Industrial Research, CSIR), currently on leave of absence at the International Institute of Tropical Agriculture (IITA).

A hard-knock life, but still going strong
In keeping with its humble heritage, cassava is a crop which has long been reputed for being more than a little worn through at the elbows, commonly known as a “poor man’s crop” according to GCP cassava breeder and researcher, Elizabeth Parkes. However, much like a dishevelled duffle coat, what the crop lacks in shimmer and shine, it makes up for in sturdiness and dependability, rising to the occasion time and again by filling a critical gap – that of putting food in bellies – with a readiness and ease that its more sophisticated crop relatives have often struggled to keep up with. Elizabeth explains:  “It has kept people alive over the years.” By the same token, the crop – now one of Africa’s most important staples – is fondly known in Ghana as bankye, meaning a ‘gift from the government’, thanks to its reliability and capacity to meet needs that other crops cannot. There is even a popular song in the country which pays homage to the crop as an indefatigable evergreen, conquering even the most willful and wily of weeds!

However, as cassava experts such as Elizabeth know only too well, behind this well-intentioned lyrical window dressing is the poignant story of a crop badly in need of a pressing pick-me-up. Hardy as it may seem on the surface, cassava is riddled with myriad problems of a political, physiological, environmental and socioeconomic nature, further compounded by the interactions between these. For starters, while it may be a timeless classic and a must-have item at the family table for a good part of Africa, à la mode it is not, or at least not for short-sighted policy-makers looking first and foremost to tighten their purse strings in straitened times, or for quick-fix, rapid-impact,  silver-bullet solutions: “African governments don’t invest many resources in research. Money is so meager, and funds have mostly come from external agencies looking to develop major cereals such as rice. Cassava has been ignored and has suffered a handicap as a result – it’s more or less an orphan crop now,” Elizabeth laments. Besides having to bear witness to their favourite outfit being left on the funding shelf, cassava breeders such as Elizabeth are also faced with a hotchpotch of hurdles in the field: “In addition to factors such as pests and disease, cassava is a long-season and very labour-intensive crop. It can take a whole year before you can expect to reap any rewards, and if you don’t have a strong team who can step in at different points throughout the breeding  process, you can often find unexpected results at the end of it, and then you have to start all over again,” Elizabeth reveals. Robust as it may be, then, cassava is no easy customer in the field: “After making crosses, you don’t have many seeds to move you to the next level, simply because with cassava, you just don’t get the numbers: some are not compatible, some are not flowering; it’s a real bottleneck that needs to be overcome,” she affirms.

No time for skirting the issue
And at the ready to flex their research muscles and rise to these considerable challenges was Elizabeth and her Ghanaian CRI  team, who – with GCP support and in unison with colleagues from across Africa and the wider GCP cassava community – have been working flat out to put cassava firmly back on the research runway.

Thanks to funders such as GCP, who recognised that we couldn’t afford to turn a blind eye to the plight of this struggling crop, cassava has been given a voice…cassava is no longer just a poor man’s staple” 

A cassava farmer in Northern Ghana.

A  cassava farmer in Northern Ghana.

Elizabeth walks us through the team’s game plan: “GCP socioeconomist Glenn Hyman and team undertook a study to identify the best area in Ghana for supporting cassava flowering [Editor’s note: Glenn works at the International Center for Tropical Agriculture, CIAT]. Armed with that information, we have been applying grafting techniques, using hormones to induce flowering in Ghana and beyond.” The initiative is starting to bear fruit: “At the IITA–Nigeria Ubiaja site, for example, flowering is underway at factory-like efficiency – it’s a great asset. The soil has also greatly improved – we haven’t been able to pinpoint the exact cause yet, but what we’ve seen is that all cultivars there will now flower,” she reveals. Elizabeth’s team has been making steady progress in biotechnological techniques such as DNA extraction: thanks to work led by then GCP cassava comrade Martin Fregene (then with the International Center for Tropical Agriculture, CIAT, and now with the Donald Danforth Plant Science Center) and colleagues, focusing on the development of more reliable and robust simple sequence repeat (SSR) markers, Elizabeth was able to carry out genetic diversity diagnosis work on cassava, collecting germplasm from all over Ghana for the global GCP cassava reference set. [Editor’s note: A ‘reference set’ is a sub-sample of existing germplasm collections that facilitates and enables access to existing crop diversity for desired traits, such as drought tolerance or resistance to disease or pests]

Similar work was also conducted in Nigeria and Guatemala. So has this tremendous and tenacious teamwork proved strong enough to drag cassava out of the doldrums? Elizabeth certainly seems to think so: “Thanks to funders such as GCP, who recognised that we couldn’t afford to turn a blind eye to the plight of this struggling crop, cassava has been given a voice. Having worked together to understand the peculiarities of this crop, cassava is no longer just a poor man’s staple: beyond subsistence, it is becoming a crop of high starch quality, and of real use for industry, confectionary and even biofuels,” she enthuses.

Thankfully, it’s a most welcome change of tide that shows no sign of abating any time soon.  Human capacity, Elizabeth says, is going from strength to strength, with three GCP-funded Ghanaian postgraduate students advancing well, two of them working on PhDs in what would normally be considered, according to Elizabeth, a ‘no-go area’ of cassava research – that is, cassava drought tolerance and post-harvest physiological deterioration (PPD), as well as bio-fortification. Efforts by the CRI team have resulted in the release of some 14–15 new drought-tolerant and PPD-resistant varieties in Ghana to date; all are anticipated to have a long shelf-life, and other varieties are also in the pipeline. Biofortified seeds are in the making, with a view to soon mainstream biofortification in the team’s breeding activities. The biofortification work is in collaboration with a sister CGIAR Challenge Programme, HarvestPlus.

The impact of our GCP-supported research on cassava has been remarkable. Above all, it’s been the community spirit which has moved things forward so effectively; in this respect, I think researchers working on other crops might want to borrow a leaf from the cassava book!”

Molecular masterstrokes, a leaf to lend despite cold shoulder, and a ‘challenge crop’ befitting Challenge Programmes
Forthcoming plans for Elizabeth and her cassava companions in Ghana include a GCP Cassava Challenge Initiative project which will seek to unearth new marker populations and materials which are drought-tolerant and resistant to cassava mosaic virus and cassava bacterial blight. The team has successfully introgressed materials from CIAT into their landraces, and the next step will be to gauge how best the new genes will react to these traits of interest. In terms of people power, the CRI biotechnology laboratory built with GCP support – and now a regionally accredited ‘Centre of Excellence’ – is a hive of activity for local and international scientists alike, and is consequently bolstering cassava research efforts in the wider subregion. “The impact of our GCP-supported research on cassava has been remarkable. Above all, it’s been the community spirit which has moved things forward so effectively; in this respect, I think researchers working on other crops might want to borrow a leaf from the cassava book!” Elizabeth ventures.

Reflecting back on the conspicuous cocktail of constraints which mired the crop in the early days of her research career – challenges which often resulted in a cold shoulder from many of her research peers over the years – Elizabeth recalls affectionately: “At first, people didn’t want to work on cassava since it’s truly a challenge crop: the genetics of cassava are really tricky. Colleagues from around the globe often asked me: ‘Why not go for a smooth crop which is friendly and easy?’” Her commitment, however, has been unfaltering throughout: “I’ve stuck with cassava because that’s my destiny! And now I see SNPs being developed, as well as numerous other resources. Once you clean something up it becomes more attractive, and my thanks go out to all those who’ve remained dedicated and helped us to achieve this.”

Thus, dusted down and  ‘marked-up’ with a molecular make-over well underway, all evidence now suggests that this once old-hat subsistence crop is en route to becoming the next season’s big research hit, with shiny new cassava varieties soon to be released at a field station near you! Go, Ghana, go!

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